MCM10 Knockout cell line(KYSE-30)

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MCM10 Knockout cell line(KYSE-30) Order

MCM10 Knockout cell line(KYSE-30)
Catalog#
YKO-HJ089
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
MCM10 Knockout cell line(KYSE-30)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
MCM10 Knockout cell line (KYSE-30)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:2~1:4
Species
Human
Gene Name
MCM10
Gene ID
55388
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
40%RPMI-1640+40%F12K+10%FBS+1% Glutamine
Accession
CVCL_E0X0(Cellosaurus)
ExPASy Cellosaurus Database
Is a valuable online resource that compiles detailed information on over 100,000 cell lines from reputable cell banks such as ATCC, ECACC, and DSMZ. The database provides information including descriptions, origins, and characteristics of the cell lines, with broad reference value and scientific reliability. Additionally, the database provides information on cell line authentication, STR profiles, and potential contamination risks, helping researchers ensure the trustworthiness of the cell lines used in their experiments.
Target region
Knockout region: Exon 3 (based on transcript MCM10-202)
Validation: PCR+Sanger Sequencing
MCM10 Knockout cell line(KYSE-30) Target region
STR Profile
Click to download the STR appraisal report
Loci STR Info (Sample Cell)
Sample Cell Line:KYSE-30		 STR Info (Cell bank)
Cell Line:KYSE-30
Allele1Allele2Allele3Allele1Allele2Allele3
D5S818 11111111
D13S317 9999
D7S820 1111.31111.3
D16S539 10121012
VWA 161819.0161819.0
TH01 9999
AMEL XXXX
TPOX 9999
CSF1PO 10101010
D12S391 1719
FGA 2424
D2S1338 2323
D21S11 2828
D18S51 1414
D8S1179 1215
D3S1358 1516
D6S1043 1120
PENTAE 1313
D19S433 14.215.2
PENTAD 1212
D1S1656 1516
* STR authentication data of this cell line matches with that of cell lines sourced from ATCC, DSMZ, JCRB, and RIKEN databases.
Conclusion: The STR identification of this cell is correct.

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the MCM10 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the KYSE-30 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, MCM10 gene knockout MCM10 Knockout cell line (KYSE-30) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
MCM10 Knockout cell line(KYSE-30)
MCM10 Knockout cell line(KYSE-30)
Catalog#
YKO-HJ089
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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